RTF1 anticorps

N° du produit ABIN7118711

Détail du produit anti-RTF1 anticorps

Antigène: RTF1 (RNA polymerase-associated protein RTF1 homolog (RTF1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RTF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: Rtf1, Paf1/RNA polymerase II complex component, homolog(S. cerevisiae)

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Date de péremption: 12 months

Détails sur RTF1

Antigène: RTF1 (RNA polymerase-associated protein RTF1 homolog (RTF1))

Autre désignation: RTF1 (RTF1 Produits)

Synonymes: anticorps CG10955, anticorps Dmel\\CG10955, anticorps dRtf1, anticorps MGC89486, anticorps RTF1, anticorps fb33g03, anticorps wu:fa04h07, anticorps wu:fb33g03, anticorps GTL7, anticorps KIAA0252, anticorps 2900005O08Rik, anticorps 6530416A09Rik, anticorps AI853581, anticorps AU043144, anticorps AW553985, anticorps Gtl7, anticorps cbp82, anticorps CG10955 gene product from transcript CG10955-RB, anticorps RTF1 homolog, Paf1/RNA polymerase II complex component, anticorps RTF1 homolog, Paf1/RNA polymerase II complex component L homeolog, anticorps RTF1, Paf1/RNA polymerase II complex component, anticorps Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae), anticorps Rtf1, anticorps rtf1, anticorps RTF1, anticorps rtf1.L

Sujet: Synonyms:KIAA0252 Background:Component of the PAF1 complex(PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'-and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1, it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4'(H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B(H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4'(H3K4me3) on genes involved in stem cell pluripotency, this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex(By similarity).

Poids moléculaire: 100 kDa

ID gène: 23168

UniProt: Q92541

Pathways: Stem Cell Maintenance