Recombinant N-Cadherin anticorps

N° du produit ABIN7504129

Détail du produit anti-N-Cadherin anticorps

Antigène: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp N-Cadherin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells.Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length human CDH2 protein

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: Human heart, pancreas or cerebral cortex (IHC).

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Date de péremption: 24 months

Détails sur N-Cadherin

Antigène: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Autre désignation: CDH2 (CDH2 Produits)

Synonymes: anticorps CD325, anticorps CDHN, anticorps CDw325, anticorps NCAD, anticorps N-cadherin, anticorps cadherin-2, anticorps cdhn, anticorps ncad, anticorps cdw325, anticorps CDH2, anticorps Cadherin-2, anticorps Ncad, anticorps glo, anticorps lyr, anticorps pac, anticorps wu:fb47h04, anticorps cadherin 2, anticorps cadherin 2 S homeolog, anticorps cadherin 2, type 1, N-cadherin (neuronal), anticorps CDH2, anticorps cdh2.S, anticorps cdh2, anticorps Cdh2

Sujet: Cadherin-2 N cadherin neuronal, Cadherin-2 type 1, Cadherin-2, Calcium dependent adhesion protein neuronal, CD325, CDH2, CDHN, CDw325, N-Cadherin, NCAD,N-Cadherin / Cadherin-2 / CD325 (NCAD)
Cellular localisation: Cell surface

Poids moléculaire: 130-140kDa

ID gène: 1000, 464829

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane