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TDRKH anticorps

TDRKH Reactivité: Humain WB, IF, IP, FACS, IHC (f) Hôte: Souris Monoclonal unconjugated
N° du produit ABIN7504177
  • Antigène Tous les produits TDRKH
    TDRKH (Tudor and KH Domain Containing (TDRKH))
    Reactivité
    • 11
    • 5
    • 5
    Humain
    Hôte
    • 8
    • 2
    • 1
    Souris
    Clonalité
    • 10
    • 1
    Monoclonal
    Conjugué
    • 9
    • 1
    • 1
    Cet anticorp TDRKH est non-conjugé
    Application
    • 10
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Specificité
    Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.
    Réactivité croisée (Details)
    Human. Predicted to react with Mouse and Rat.
    Purification
    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
    Immunogène
    Recombinant full-length human TDRKH protein
    Isotype
    IgG2b
  • Indications d'application

    Positive Control: HeLa or MCF7 cells. Human testis or brain.

    Known Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.
    Date de péremption
    24 months
  • Antigène
    TDRKH (Tudor and KH Domain Containing (TDRKH))
    Autre désignation
    TDRKH (TDRKH Produits)
    Synonymes
    anticorps RGD1311196, anticorps tdrd2, anticorps MGC85144, anticorps TDRD2, anticorps 2700091C21Rik, anticorps Tdrd2, anticorps tudor and KH domain containing, anticorps tudor and KH domain containing L homeolog, anticorps tudor and KH domain containing protein, anticorps Tdrkh, anticorps TDRKH, anticorps tdrkh.L, anticorps tdrkh
    Sujet
    2700091C21Rik, Putative RNA binding protein, RGD1311196, RP11-98D18.8, TDRD2, TDRD7, Tdrkh, Tudor and KH domain containing, Tudor and KH domain-containing protein, tudor domain containing 2,TDRKH
    Cellular localisation: Cytoplasm. Localized to cytosol and centrosome.
    Poids moléculaire
    62.05kDa
    ID gène
    11022, 144439
    UniProt
    Q9Y2W6
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