DCP2 anticorps

N° du produit ABIN7126292

Détail du produit anti-DCP2 anticorps

Antigène: DCP2 (DCP2 Decapping Enzyme Homolog (DCP2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DCP2 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificité: The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5' to 3' exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay

Réactivité croisée (Details): Human. Predicted to react with Mouse and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human DCP2 protein

Isotype: IgG2a

Information d'application

Indications d'application:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), ,Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: MCF7, Daudi or Jurkat cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur DCP2

Antigène: DCP2 (DCP2 Decapping Enzyme Homolog (DCP2))

Autre désignation: DCP2 (DCP2 Produits)

Synonymes: anticorps DDBDRAFT_0185453, anticorps DDBDRAFT_0252811, anticorps DDB_0185453, anticorps DDB_0252811, anticorps 2410015D23Rik, anticorps 5730537H01Rik, anticorps AL118268, anticorps NUDT20, anticorps RGD1562909, anticorps mRNA-decapping enzyme 2, anticorps hypothetical protein, anticorps decapping mRNA 2, anticorps CpipJ_CPIJ019463, anticorps PTRG_05936, anticorps dcp2, anticorps MCYG_05106, anticorps MGYG_07617, anticorps PGTG_03310, anticorps Tsp_04018, anticorps DCP2, anticorps Dcp2

Sujet: DCP2 decapping enzyme homolog (S. cerevisiae), DFLJ33245, hDpc, m7GpppN-mRNA hydrolase, mRNA decapping enzyme 2, nudix (nucleoside diphosphate linked moiety X)-type motif 20 (NUDT20),DCP2 (Decapping mRNA 2)
Cellular localisation: Cytoplasm > P. body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear.

Poids moléculaire: 48.4kDa

ID gène: 167227, 443875

UniProt: Q8IU60