Recombinant EPX anticorps

N° du produit ABIN7127087

Détail du produit anti-EPX anticorps

Antigène: EPX (Eosinophil Peroxidase (EPX))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EPX est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Human eosinophils from a patient with hypereosinophilic syndrome

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Tonsil

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur EPX

Antigène: EPX (Eosinophil Peroxidase (EPX))

Autre désignation: EPX (EPX Produits)

Synonymes: anticorps EPX, anticorps pmr-1, anticorps EPO, anticorps EPP, anticorps EPX-PEN, anticorps eosinophil peroxidase, anticorps eosinophil peroxidase L homeolog, anticorps LOC788751, anticorps EPX, anticorps epx.L, anticorps Epx

Sujet: Eosinophil peroxidase heavy chain, EPER, EPO, EPP, EPX, EPX PEN, PERE,Eosinophil Peroxidase (EPX)
Cellular localisation: Cytoplasmic, granular

Poids moléculaire: 70kDa (55kDa-Heavy Chain, 15kDa-Light Chain)

ID gène: 8288, 279259

UniProt: P11678