Recombinant Histone H3.3 anticorps

N° du produit ABIN7127093

Détail du produit anti-Histone H3.3 anticorps

Antigène: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone H3.3 est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3 was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clark's level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: A synthetic peptide corresponding to (ARK-pS-TGGKAPRKQLc) of Phosphohistone H3 (phospho S10)

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, liver or breast carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Histone H3.3

Antigène: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Autre désignation: H3F3 (H3F3A Produits)

Synonymes: anticorps H3.3A, anticorps H3F3, anticorps h3f3a, anticorps MGC52829, anticorps wu:fb58e10, anticorps zgc:56193, anticorps zgc:86731, anticorps H3-I, anticorps H3-II, anticorps H3-III, anticorps H3-IV, anticorps H3-V, anticorps H3-VI, anticorps H3-VII, anticorps H3-VIII, anticorps H3F3A, anticorps h3.3, anticorps h3.3a, anticorps CG5825, anticorps Dmel\\CG5825, anticorps H3.3, anticorps His-3.3A, anticorps His3.3, anticorps PH3, anticorps dH3.3A, anticorps H3 histone family member 3A, anticorps H3 histone, family 3A, anticorps H3 histone, family 3A L homeolog, anticorps H3 histone, family 3C, anticorps histone H3.3, anticorps histone cluster 1, H3a, anticorps Histone H3.3A, anticorps H3F3A, anticorps h3f3a.L, anticorps h3f3a, anticorps H3F3C, anticorps LOC475916, anticorps LOC480110, anticorps HIST1H3A, anticorps LOAG_05159, anticorps His3.3A, anticorps H3f3a

Sujet: Hist1h3a, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, Histone cluster 1, H3a, Histone H3 3 pseudogene, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l,Histone H3, phosphorylated (pSer10) (Nuclear Marker)
Cellular localisation: Nucleus. Chromosome.

Poids moléculaire: 15kDa

ID gène: 8350, 132854

UniProt: P68431