MED21 anticorps

N° du produit ABIN7127181

Détail du produit anti-MED21 anticorps

Antigène: MED21 (Mediator Complex Subunit 21 (MED21))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MED21 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificité: In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase. These complexes include the SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes and including the human proteins Srb7, Med6 (also designated DRIP33) and Med7 (also designated DRIP34). SMCC associates with the RNAPII (RNA polymerase II) holoenzyme through Srb7 and, in turn, enhances gene-specific activation or repression induced by DNA-binding transcription factors. Med6 and Med7, as well as other components of SMCC, associate with co-activator proteins from the TRAP (thyroid hormone receptor-activating protein) complex and DRIP (for vitamin D receptor interacting protein) complex to facilitate steroid receptor dependent transcriptional activation. Additionally, SMCC associates with PC4 (positive cofactor 4) to repress basal transcription independent of RNAPII activity

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human MED21 protein

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or K562 cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur MED21

Antigène: MED21 (Mediator Complex Subunit 21 (MED21))

Autre désignation: MED21 (MED21 Produits)

Synonymes: anticorps SURB7, anticorps fk48d02, anticorps surb7, anticorps wu:fk48d02, anticorps zgc:56532, anticorps SRB7, anticorps hSrb7, anticorps 0610007L03Rik, anticorps AI449604, anticorps D19234, anticorps D6Ertd782e, anticorps Srb7, anticorps Surb7, anticorps T4B21.8, anticorps T4B21_8, anticorps mediator 21, anticorps mediator complex subunit 21, anticorps Med21p, anticorps mediator 21, anticorps MED21, anticorps med21, anticorps Med21

Sujet: Forkhead box protein Q1, MED21, Hepatocyte nuclear factor 3 forkhead homolog 1, HFH1, HNF3/forkhead like protein 1, MGC77469, Q1, Winged helix / forkhead transcription factor, zgc:77469,MED21 / SRB7
Cellular localisation: Nucleus. Nucleoplasm. Cytoplasm.

Poids moléculaire: 15.5kDa

ID gène: 9412, 286145

UniProt: Q13503

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha