Recombinant ACTA1/ACTA2A/ACTG2 anticorps

N° du produit ABIN7127250

Détail du produit anti-ACTA1/ACTA2A/ACTG2 anticorps

Antigène: ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ACTA1/ACTA2A/ACTG2 est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)

Specificité: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Réactivité croisée (Details): Human, Rabbit and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant protein corresponding to human muscle actin

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Western Blot (0.5-1.0 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Muscle or sarcoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur ACTA1/ACTA2A/ACTG2

Antigène: ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))

Abstract: ACTA1/ACTA2A/ACTG2 Produits

Sujet: ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1,Actin, Muscle Specific (Muscle Cell Marker)
Cellular localisation: Cytoplasm.

Poids moléculaire: 42kDa

ID gène: 58, 59, 70

UniProt: P62736, P68032, P68133