Recombinant BRD4 anticorps

N° du produit ABIN7127371

Détail du produit anti-BRD4 anticorps

Antigène: BRD4 (Bromodomain Containing 4 (BRD4))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp BRD4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Réactivité croisée: Humain

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human Brd4

Clone: 1E5

Isotype: IgG

Information d'application

Indications d'application: Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200,

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur BRD4

Antigène: BRD4 (Bromodomain Containing 4 (BRD4))

Autre désignation: BRD4 (BRD4 Produits)

Synonymes: anticorps brd4-b, anticorps BRD4, anticorps fb18g03, anticorps wu:fb18g03, anticorps CAP, anticorps HUNK1, anticorps HUNKI, anticorps MCAP, anticorps Brd5, anticorps WI-11513, anticorps bromodomain containing 4 L homeolog, anticorps bromodomain containing 4, anticorps bromodomain-containing protein 4, anticorps brd4.L, anticorps BRD4, anticorps brd4, anticorps LOC100409351, anticorps Brd4

Sujet:

Background: Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II, these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.

Aliases: Bromodomain-containing protein 4 (Protein HUNK1), BRD4, HUNK1

UniProt: O60885

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome