Recombinant DGCR8 anticorps

N° du produit ABIN7127453

Détail du produit anti-DGCR8 anticorps

Antigène: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp DGCR8 est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée: Humain

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human DGCR8

Clone: 2H2

Isotype: IgG

Information d'application

Indications d'application: Recommended dilution: WB:1:500-1:5000,

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur DGCR8

Antigène: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Autre désignation: DGCR8 (DGCR8 Produits)

Synonymes: anticorps MGC78846, anticorps DGCR8, anticorps gy1, anticorps dgcrk6, anticorps C22orf12, anticorps DGCRK6, anticorps Gy1, anticorps pasha, anticorps D16H22S1742E, anticorps D16H22S788E, anticorps D16Wis2, anticorps N41, anticorps Vo59c07, anticorps si:ch211-106a19.4, anticorps wu:fc23f08, anticorps wu:fc38f06, anticorps DGCR8 microprocessor complex subunit L homeolog, anticorps DiGeorge syndrome critical region gene 8, anticorps DGCR8 microprocessor complex subunit, anticorps DGCR8, microprocessor complex subunit, anticorps microRNA 3618, anticorps dgcr8.L, anticorps DGCR8, anticorps dgcr8, anticorps Dgcr8, anticorps MIR3618

Sujet:

Background: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity).

Aliases: Microprocessor complex subunit DGCR8, DiGeorge syndrome critical region 8, DGCR8, C22orf12, DGCRK6, LP4941

UniProt: Q8WYQ5

Pathways: Regulatory RNA Pathways