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MCAM anticorps (AA 50-646)

Name: MCAM anticorps (AA 50-646)
Brand: antibodies-online
SKU: ABIN7147325
Price: 385 EUR
Availability: InStock

MCAM Reactivité: Humain FACS, WB, IF, ELISA Hôte: Souris Monoclonal 4D10C7 unconjugated

Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CD146 antibody at 1:1250 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 72, 58 kDa Observed band size: 120 kDa (MCAM anticorps (AA 50-646))

Overlay histogram showing MCF-7 cells stained with ABIN7147325 (red line) at 1:400. (MCAM anticorps (AA 50-646))

FACS

Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate All lanes: CD146 antibody at 1:500 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 72, 58 kDa Observed band size: 120 kDa (MCAM anticorps (AA 50-646))

Immunofluorescence staining of A375 cells with ABIN7147325 at 1:100, counter-stained with DAPI. (MCAM anticorps (AA 50-646))

Immunofluorescence staining of Hela cells with ABIN7147325 at 1:100, counter-stained with DAPI. (MCAM anticorps (AA 50-646))

Immunofluorescence staining of MCF-7 cells with ABIN7147325 at 1:100, counter-stained with DAPI. (MCAM anticorps (AA 50-646))

Immunofluorescence staining of THP-1 cells with ABIN7147325 at 1:100, counter-stained with DAPI. (MCAM anticorps (AA 50-646))

7 images

N° du produit ABIN7147325

Fiche de données

Antigène Voir toutes MCAM Anticorps

MCAM (Melanoma Cell Adhesion Molecule (MCAM))
Épitope
16

13

11

5

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 50-646
Reactivité
133

53

47

14

14

9

8

7

6

2
Humain
Hôte
95

62

8

1

1
Souris
Anticorps secondaires appropriés
Clonalité
88

79
Monoclonal
Conjugué
70

14

14

11

6

5

4

4

4

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp MCAM est non-conjugé
Application
85

79

69

42

21

20

14

10

10

5

4

4

3

3

2

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), ELISA

Réactivité croisée

Humain

Purification

Protein G purified

Immunogène

Recombinant Human Cell surface glycoprotein MUC18 protein (50-646AA)

Clone

4D10C7

Isotype

IgG2a

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Indications d'application

Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C,-80 °C

Stockage commentaire

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antigène

MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Autre désignation

CD146 (MCAM Produits)

Synonymes

anticorps CD146, anticorps MUC18, anticorps Muc18, anticorps 1-gicerin, anticorps AV025631, anticorps CD149, anticorps s-endo, anticorps s-gicerin, anticorps cd146, anticorps gicerin, anticorps id:ibd3512, anticorps mcam, anticorps MCAM, anticorps melanoma cell adhesion molecule, anticorps melanoma cell adhesion molecule b, anticorps MCAM, anticorps Mcam, anticorps mcamb

Sujet

Background: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Aliases: Cell surface glycoprotein MUC18 (Cell surface glycoprotein P1H12) (Melanoma cell adhesion molecule) (Melanoma-associated antigen A32) (Melanoma-associated antigen MUC18) (S-endo 1 endothelial-associated antigen) (CD antigen CD146), MCAM, MUC18

UniProt

P43121