MLH1 anticorps (AA 350-519)

N° du produit ABIN7150201

Détail du produit anti-MLH1 anticorps

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Épitope: AA 350-519

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MLH1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Réactivité croisée: Humain

Purification: >95%, Protein G purified

Immunogène: Recombinant Human DNA mismatch repair protein Mlh1 protein (350-519AA)

Isotype: IgG

Information d'application

Indications d'application: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200,

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur MLH1

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Autre désignation: MLH1 (MLH1 Produits)

Synonymes: anticorps CG11482, anticorps Dmel\\CG11482, anticorps dmlh-1, anticorps dmlh1, anticorps zgc:66301, anticorps MLH1, anticorps LOC100232198, anticorps 1110035C23Rik, anticorps AI317206, anticorps AI325952, anticorps AI561766, anticorps COCA2, anticorps FCC2, anticorps HNPCC, anticorps HNPCC2, anticorps hMLH1, anticorps mutL homolog 1, anticorps CG11482 gene product from transcript CG11482-RB, anticorps mutL homolog 1 S homeolog, anticorps mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), anticorps DNA mismatch repair protein Mlh1, anticorps MLH1, anticorps Mlh1, anticorps mlh1.S, anticorps mlh1, anticorps LOC588545

Sujet:

Background: Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Aliases: COCA 2 antibody, COCA2 antibody, DNA mismatch repair protein Mlh1 antibody, FCC 2 antibody, FCC2 antibody, hMLH 1 antibody, hMLH1 antibody, HNPCC 2 antibody, HNPCC antibody, HNPCC2 antibody, MGC5172 antibody, MLH 1 antibody, MLH1 antibody, MLH1_HUMAN antibody, MutL homolog 1 (E. coli) antibody, MutL homolog 1 antibody, MutL homolog 1 colon cancer nonpolyposis type 2 antibody, MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) antibody, MutL protein homolog 1 antibody, MutL, E. coli, homolog of, 1 antibody

UniProt: P40692

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response