Topoisomerase I anticorps (AA 358-501)

N° du produit ABIN7150342

Détail du produit anti-Topoisomerase I anticorps

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Épitope: AA 358-501

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Topoisomerase I est non-conjugé

Application: ELISA, Immunofluorescence (IF)

Réactivité croisée: Humain

Purification: >95%, Protein G purified

Immunogène: Recombinant Human DNA topoisomerase 1 protein (358-501AA)

Isotype: IgG

Information d'application

Indications d'application: Recommended dilution: IF:1:50-1:200,

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur Topoisomerase I

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Autre désignation: TOP1 (TOP1 Produits)

Classe de substances: Viral Protein

Sujet:

Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Aliases: DNA topoisomerase 1 antibody, DNA topoisomerase I antibody, NUP98 fusion gene antibody, TOP 1 antibody, TOP I antibody, TOP1 antibody, TOP1_HUMAN antibody, TOPI antibody, Topoisomerase (DNA) I antibody, Topoisomerase 1 antibody, Topoisomerase1 antibody, TopoisomeraseI antibody, Type I DNA topoisomerase antibody

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance