Pour une meilleure utilisation, nous vous conseillons d’activer Javascript dans votre navigateur.

Hsc70 anticorps (AA 2-646)

Name: Hsc70 anticorps (AA 2-646)
Brand: antibodies-online
SKU: ABIN7154884
Price: 385 EUR
Availability: InStock

HSPA8 Reactivité: Humain WB, IHC, ELISA, IF, FACS Hôte: Souris Monoclonal 6E4D2 unconjugated

Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate All lanes HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 5 min (Hsc70 anticorps (AA 2-646))

Overlay histogram showing MCF-7 cells stained with ABIN7154884 (red line). (Hsc70 anticorps (AA 2-646))

FACS

Western Blot Positive WB detected in: Hela whole cell lysate at 20 μg, 10 μg, 5 μg, 2. (Hsc70 anticorps (AA 2-646))

Western Blot Positive WB detected in: 20 μg hela whole cell lysate HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 5 min (Hsc70 anticorps (AA 2-646))

IHC image of ABIN7154884 diluted at 1:220 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. (Hsc70 anticorps (AA 2-646))

IHC

IHC image of ABIN7154884 diluted at 1:220 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. (Hsc70 anticorps (AA 2-646))

IHC

Immunofluorescence staining of Hela cells with ABIN7154884 at 1:100, counter-stained with DAPI. (Hsc70 anticorps (AA 2-646))

Immunofluorescence staining of MCF-7 cells with ABIN7154884 at 1:100, counter-stained with DAPI. (Hsc70 anticorps (AA 2-646))

Immunofluorescence staining of PC-3 cells with ABIN7154884 at 1:100, counter-stained with DAPI. (Hsc70 anticorps (AA 2-646))

9 images

N° du produit ABIN7154884

Fiche de données

Antigène Voir toutes Hsc70 (HSPA8) Anticorps

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
Épitope
14

7

6

5

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-646
Reactivité
81

48

44

21

17

14

13

11

11

11

10

10

5

5

5

4

4

3

3

3

2

1

1

1

1

1

1

1
Humain
Hôte
70

27

7

2
Souris
Anticorps secondaires appropriés
Clonalité
70

35
Monoclonal
Conjugué
58

19

11

3

3

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp Hsc70 est non-conjugé
Application
92

51

39

29

29

27

15

13

11

11

11

5

4

2

2

2

2

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Réactivité croisée

Humain

Purification

Protein G purified

Immunogène

Recombinant Human Heat shock cognate 71 kDa protein (2-646AA)

Clone

6E4D2

Isotype

IgG1, IgG1 kappa
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 Reactivité: Humain WB, IHC, ELISA, IP, FACS Hôte: Souris Monoclonal 2G8F6 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibody
HSPA8 Reactivité: Humain, Rat, Souris WB, ICC, IF, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 1-300) antibody
HSPA8 Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 82-110), (N-Term) antibody
HSPA8 Reactivité: Humain WB, IF, IHC (p), FACS Hôte: Lapin Polyclonal RB17655 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 539-569), (C-Term) antibody
HSPA8 Reactivité: Humain WB, IF, IHC (p), FACS Hôte: Lapin Polyclonal RB16528 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody
HSPA8 Reactivité: Humain WB, IHC, ELISA, IP, ICC, IF, PLA, Bind, AA Hôte: Souris Monoclonal 1F2-H5 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 13-31), (N-Term) antibody
HSPA8 Reactivité: Humain, Rat, Souris WB, ICC, IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibody
HSPA8 Reactivité: Humain, Rat, Souris, Boeuf (Vache), Mouton, Porc, Poisson zèbre (Danio rerio), Chévre WB, IHC Hôte: Lapin Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 Reactivité: Souris WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 418-597) antibody
HSPA8 Reactivité: Humain WB, IHC, ELISA Hôte: Lapin Polyclonal unconjugated

Indications d'application

Recommended dilution: WB: 1:5000-1:32000, IHC: 1:100-1:500, IF: 1:100-1:300, FC: 1:100-1:300,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C,-80 °C

Stockage commentaire

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antigène

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Autre désignation

HSPA8 (HSPA8 Produits)

Synonymes

anticorps hsc54, anticorps hsc70, anticorps hsc71, anticorps hsp71, anticorps hsp73, anticorps hspa10, anticorps lap1, anticorps nip71, anticorps HSC54, anticorps HSC70, anticorps HSC71, anticorps HSP71, anticorps HSP73, anticorps HSPA10, anticorps LAP1, anticorps NIP71, anticorps Hsc70, anticorps 2410008N15Rik, anticorps Hsc71, anticorps Hsc73, anticorps Hsp73, anticorps Hspa10, anticorps wu:fb01g06, anticorps wu:fi48b06, anticorps heat shock protein family A (Hsp70) member 8 L homeolog, anticorps heat shock protein family A (Hsp70) member 8, anticorps heat shock 70kDa protein 8, anticorps heat shock protein 8, anticorps hspa8.L, anticorps HSPA8, anticorps Hspa8, anticorps hspa8

Sujet

Background: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP
Aliases: Heat shock cognate 71 kDa protein (Heat shock 70 kDa protein 8) (Lipopolysaccharide-associated protein 1) (LAP-1) (LPS-associated protein 1), HSPA8, HSC70 HSP73 HSPA10

UniProt

P11142