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Hsc70 anticorps (AA 2-646)

Name: Hsc70 anticorps (AA 2-646)
Brand: antibodies-online
SKU: ABIN7154884
Price: 462 EUR
Availability: InStock

HSPA8 Reactivité: Humain WB, IHC, ELISA, IF, FACS Hôte: Souris Monoclonal 6E4D2 unconjugated

Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate All lanes HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 5 min (Hsc70 anticorps (AA 2-646))

Overlay histogram showing MCF-7 cells stained with ABIN7154884 (red line). (Hsc70 anticorps (AA 2-646))

FACS

Western Blot Positive WB detected in: Hela whole cell lysate at 20 μg, 10 μg, 5 μg, 2. (Hsc70 anticorps (AA 2-646))

Western Blot Positive WB detected in: 20 μg hela whole cell lysate HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 5 min (Hsc70 anticorps (AA 2-646))

IHC image of ABIN7154884 diluted at 1:220 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. (Hsc70 anticorps (AA 2-646))

IHC

IHC image of ABIN7154884 diluted at 1:220 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. (Hsc70 anticorps (AA 2-646))

IHC

Immunofluorescence staining of Hela cells with ABIN7154884 at 1:100, counter-stained with DAPI. (Hsc70 anticorps (AA 2-646))

Immunofluorescence staining of MCF-7 cells with ABIN7154884 at 1:100, counter-stained with DAPI. (Hsc70 anticorps (AA 2-646))

Immunofluorescence staining of PC-3 cells with ABIN7154884 at 1:100, counter-stained with DAPI. (Hsc70 anticorps (AA 2-646))

9 images

N° du produit ABIN7154884

Fiche de données

Antigène Voir toutes Hsc70 (HSPA8) Anticorps

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
Épitope
14

7

6

4

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-646
Reactivité
82

47

43

21

17

14

13

11

11

11

10

10

5

5

5

4

4

3

3

3

2

1

1

1

1

1

1

1
Humain
Hôte
71

27

7

2
Souris
Anticorps secondaires appropriés
Clonalité
68

38
Monoclonal
Conjugué
59

19

11

3

3

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp Hsc70 est non-conjugé
Application
93

50

39

30

29

28

16

13

11

11

11

5

4

2

2

2

2
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Réactivité croisée

Humain

Purification

Protein G purified

Immunogène

Recombinant Human Heat shock cognate 71 kDa protein (2-646AA)

Clone

6E4D2

Isotype

IgG1, IgG1 kappa
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 Reactivité: Humain WB, IHC, ELISA, IP, FACS Hôte: Souris Monoclonal 2G8F6 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibody
HSPA8 Reactivité: Humain, Rat, Souris WB, ICC, IF, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 1-300) antibody
HSPA8 Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 82-110), (N-Term) antibody
HSPA8 Reactivité: Humain WB, IF, IHC (p), FACS Hôte: Lapin Polyclonal RB17655 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 539-569), (C-Term) antibody
HSPA8 Reactivité: Humain WB, IF, IHC (p), FACS Hôte: Lapin Polyclonal RB16528 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody
HSPA8 Reactivité: Humain WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA Hôte: Souris Monoclonal 1F2-H5 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 13-31), (N-Term) antibody
HSPA8 Reactivité: Humain, Rat, Souris WB, ICC, IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibody
HSPA8 Reactivité: Humain, Rat, Souris, Boeuf (Vache), Mouton, Porc, Poisson zèbre (Danio rerio), Chévre WB, IHC Hôte: Lapin Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 Reactivité: Souris WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 418-597) antibody
HSPA8 Reactivité: Humain WB, IHC, ELISA Hôte: Lapin Polyclonal unconjugated

Indications d'application

Recommended dilution: WB: 1:5000-1:32000, IHC: 1:100-1:500, IF: 1:100-1:300, FC: 1:100-1:300,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C,-80 °C

Stockage commentaire

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antigène

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Autre désignation

HSPA8 (HSPA8 Produits)

Sujet

Background: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP
Aliases: Heat shock cognate 71 kDa protein (Heat shock 70 kDa protein 8) (Lipopolysaccharide-associated protein 1) (LAP-1) (LPS-associated protein 1), HSPA8, HSC70 HSP73 HSPA10

UniProt

P11142