PKC theta anticorps (pThr538)

N° du produit ABIN711746

Détail du produit anti-PKC theta anticorps

Antigène: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Épitope: pThr538

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC theta est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Humain, Souris

Homologie: Rat,Dog

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human PRKCQ around the phosphorylation site of Thr538

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-PKC theta (ABIN711746)

van Rees, Lago, Cox, Tomasik, Rustogi, Weigelt, Ozcan, Cooper, Drexhage, Leweke, Bahn: "Evidence of microglial activation following exposure to serum from first-onset drug-naïve schizophrenia patients." dans: Brain, behavior, and immunity, Vol. 67, pp. 364-373, (2018) (PubMed).

Détails sur PKC theta

Antigène: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Autre désignation: PRKCQ (PRKCQ Produits)

Synonymes: anticorps PRKCT, anticorps nPKC-theta, anticorps A130035A12Rik, anticorps AW494342, anticorps PKC-0, anticorps PKC-theta, anticorps PKCtheta, anticorps Pkcq, anticorps protein kinase C theta, anticorps protein kinase C eta, anticorps protein kinase C, theta, anticorps PRKCQ, anticorps PRKCH, anticorps Prkcq

Sujet:

Synonyms: PRKCT, nPKC-theta, Protein kinase C theta type, PRKCQ

Background: Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non-redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates to the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non-canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response.

ID gène: 5588

UniProt: Q04759

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling