GBL anticorps (AA 159-220)

N° du produit ABIN7171522

Détail du produit anti-GBL anticorps

Antigène: GBL (G protein beta subunit-like (GBL))

Épitope: AA 159-220

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GBL est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Réactivité croisée: Humain

Purification: >95%, Protein G purified

Immunogène: Recombinant Human Target of rapamycin complex subunit LST8 protein (159-220AA)

Isotype: IgG

Information d'application

Indications d'application: Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200,

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur GBL

Antigène: GBL (G protein beta subunit-like (GBL))

Autre désignation: MLST8 (GBL Produits)

Synonymes: anticorps gbl, anticorps lts8, anticorps GBL, anticorps GbetaL, anticorps LST8, anticorps POP3, anticorps WAT1, anticorps 0610033N12Rik, anticorps AA409454, anticorps AI505104, anticorps AI851821, anticorps Gbl, anticorps fi37e04, anticorps wu:fi37e04, anticorps zgc:55455, anticorps zgc:85668, anticorps MTOR associated protein, LST8 homolog L homeolog, anticorps MTOR associated protein, LST8 homolog, anticorps MTOR associated protein, LST8 homolog (S. cerevisiae), anticorps mlst8.L, anticorps MLST8, anticorps Mlst8, anticorps mlst8

Sujet:

Background: Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at \'Thr-389\', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 \'Ser-473\' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on \'Thr-308\' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at \'Ser-422\'. mTORC2 also modulates the phosphorylation of PRKCA on \'Ser-657\'.

Aliases: G protein beta subunit like antibody, G protein beta subunit-like antibody, Gable antibody, GbetaL antibody, GBL antibody, GBL protein antibody, LST8 antibody, LST8_HUMAN antibody, Mammalian lethal with SEC13 protein 8 antibody, MGC111011 antibody, mLST8 antibody, MTOR associated protein LST8 homolog (S. cerevisiae) antibody, POP3 antibody, POP3 homolog (S. pombe) antibody, Protein GbetaL antibody, Target of rapamycin complex subunit LST8 antibody, TORC subunit LST8 antibody, WAT1 antibody, WAT1 homolog (S. pombe) antibody

UniProt: Q9BVC4

Pathways: Signalisation PI3K-Akt, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, L'effet Warburg