RAG2 anticorps (AA 296-415)

N° du produit ABIN7175270

Détail du produit anti-RAG2 anticorps

Antigène: RAG2 (Recombination Activating Gene 2 (RAG2))

Épitope: AA 296-415

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAG2 est non-conjugé

Application: ELISA, Immunofluorescence (IF)

Réactivité croisée: Humain

Purification: >95%, Protein G purified

Immunogène: Recombinant Human V(D)J recombination-activating protein 2 protein (296-415AA)

Isotype: IgG

Information d'application

Indications d'application: Recommended dilution: IF:1:50-1:200,

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur RAG2

Antigène: RAG2 (Recombination Activating Gene 2 (RAG2))

Autre désignation: RAG2 (RAG2 Produits)

Synonymes: anticorps RAG-2, anticorps Rag-2, anticorps rag-2, anticorps zgc:136743, anticorps recombination activating 2, anticorps recombination activating gene 2, anticorps recombination activating gene 2 S homeolog, anticorps RAG2, anticorps Rag2, anticorps rag2, anticorps rag2.S

Sujet:

Background: Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3\'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5\'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at \'Lys-4\' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 (By similarity).

Aliases: RAG 2 antibody, RAG-2 antibody, RAG2 antibody, RAG2_HUMAN antibody, Recombination activating gene 2 antibody, V(D)J recombination activating protein 2 antibody, V(D)J recombination-activating protein 2 antibody

UniProt: P55895

Pathways: Chromatin Binding, Production of Molecular Mediator of Immune Response