ITCH anticorps (Tyr420)

N° du produit ABIN7180034

Détail du produit anti-ITCH anticorps

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Épitope: Tyr420

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ITCH est non-conjugé

Application: ELISA, Western Blotting (WB)

Réactivité croisée: Humain, Souris

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized non-phosphopeptide derived from Human ITCH around the phosphorylation site of tyrosine 420 (F-I-Y(p)-G-N).

Isotype: IgG

Information d'application

Indications d'application: WB:1:500-1:3000,

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur ITCH

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Autre désignation: ITCH (ITCH Produits)

Synonymes: anticorps AIF4, anticorps AIP4, anticorps NAPP1, anticorps dJ468O1.1, anticorps 6720481N21Rik, anticorps 8030492O04Rik, anticorps A130065M08, anticorps C230047C07Rik, anticorps itchy E3 ubiquitin protein ligase, anticorps itchy, E3 ubiquitin protein ligase, anticorps ITCH, anticorps Itch

Sujet:

Background: Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. It is involved in the control of inflammatory signaling pathways. Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages. Critical regulator of T-helper (TH2) cytokine development through its ability to induce JUNB ubiquitination and degradation By similarity. Ubiquitinates SNX9. Ubiquitinates CXCR4 and HGS/HRS and regulates sorting of CXCR4 to the degradative pathway. It is involved in the negative regulation of MAVS-dependent cellular antiviral responses. Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteasomal degradation. Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP. Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID. Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination.

Chen X., Genomics 73:238-241(2001).
Deloukas P., Nature 414:865-871(2001).
Wood J.D., Mol. Cell. Neurosci. 11:149-160(1998).

Aliases: ADMFD antibody, AIF4 antibody, AIP4 antibody, Atrophin 1 interacting protein 4 antibody, Atrophin-1-interacting protein 4 antibody, dJ468O1.1 antibody, dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)) antibody, dJ468O1.1 atrophin 1 interacting protein 4 AIP4 antibody, E3 ubiquitin protein ligase Itchy homolog antibody, E3 ubiquitin-protein ligase Itchy homolog antibody, EC 6.3.2 antibody, Itch antibody, ITCH_HUMAN antibody, Itchy E3 ubiquitin protein ligase antibody, Itchy E3 ubiquitin protein ligase homolog antibody, Itchy E3 ubiquitin protein ligase homolog mouse antibody, Itchy E3 ubiquitin protein ligase, mouse, homolog of antibody, Itchy homolog E3 ubiquitin protein ligase antibody, Itchy mouse homolog E3 ubiquitin protein ligase antibody, NAPP1 antibody, NFE2 associated polypeptide 1 antibody, NFE2-associated polypeptide 1 antibody, Ubiquitin protein ligase ITCH antibody

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events