POLA1 anticorps (N-Term)

N° du produit ABIN7184445

Détail du produit anti-POLA1 anticorps

Antigène: POLA1 (DNA Polymerase alpha (POLA1))

Épitope: N-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLA1 est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée: Humain

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from N-terminal of Human DNA Polymerase alpha.

Isotype: IgG

Information d'application

Indications d'application: WB:1:500-1:3000,

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur POLA1

Antigène: POLA1 (DNA Polymerase alpha (POLA1))

Autre désignation: POLA1 (POLA1 Produits)

Synonymes: anticorps pola, anticorps AW321876, anticorps Pola, anticorps POLA, anticorps p180, anticorps polymerase (DNA directed), alpha 1, catalytic subunit S homeolog, anticorps polymerase (DNA directed), alpha 1, anticorps DNA polymerase alpha 1, catalytic subunit, anticorps pola1.S, anticorps Pola1, anticorps POLA1

Sujet:

Background: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

Wong S.W., EMBO J. 7:37-47(1988).
Pearson B.E., Mol. Cell. Biol. 11:2081-2095(1991).
Hsi K.-L., Nucleic Acids Res. 18:6231-6237(1990).

Aliases: DKFZp686K1672 antibody, DNA polymerase alpha 1 catalytic subunit antibody, DNA polymerase alpha catalytic subunit antibody, DNA polymerase alpha catalytic subunit p180 antibody, DNA polymerase alpha p180 subunit antibody, DPOLA_HUMAN antibody, OTTHUMP00000023089 antibody, OTTHUMP00000023090 antibody, P180 antibody, p180 subunit antibody, POLA antibody, POLA1 antibody, Polymerase (DNA directed), alpha 1, catalytic subunit antibody, Polymerase (DNA-directed), alpha (70kD) antibody

UniProt: P09884

Pathways: SARS-CoV-2 Protein Interactome