EAF1 anticorps (AA 50-120)

N° du produit ABIN7266919

Détail du produit anti-EAF1 anticorps

Antigène: EAF1 (ELL Associated Factor 1 (EAF1))

Épitope: AA 50-120

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EAF1 est non-conjugé

Application: Western Blotting (WB)

Fonction: EAF1 Rabbit pAb

Séquence: LQVGKGDEVT ITLPHIPGST PPMTVFKGNK RPYQKDCVLI INHDTGEYVL EKLSSSIQVK KTRAEGSSKI Q

Réactivité croisée: Humain, Rat

Attributs du produit: Polyclonal Antibodies

Purification: Affinity purification

Immunogène: Recombinant fusion protein containing a sequence corresponding to amino acids 50-120 of human EAF1 (NP_149074.3).

Isotype: IgG

Information d'application

Indications d'application: WB,1:500 - 1:2000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze / thaw cycles.

Détails sur EAF1

Antigène: EAF1 (ELL Associated Factor 1 (EAF1))

Autre désignation: EAF1 (EAF1 Produits)

Synonymes: anticorps 4933403C17Rik, anticorps AI666536, anticorps ELL associated factor 1, anticorps EAF1, anticorps Eaf1

Sujet: Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.,EAF1,Epigenetics & Nuclear Signaling,EAF1

Poids moléculaire: 17kDa/29kDa

ID gène: 85403

UniProt: Q96JC9