SEBOV GP anticorps (AA 1-637)

N° du produit ABIN7383897

Détail du produit anti-SEBOV GP anticorps

Antigène: SEBOV GP (Sudan Ebola Virus Envelope Glycoprotein (SEBOV GP))

Épitope: AA 1-637

Reactivité: Sudan ebolavirus

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SEBOV GP est non-conjugé

Application: Western Blotting (WB)

Specificité: Anti-Ebola virus EBOV(Subtype Sudan, strain Gulu) Glycoprotein/GP Polyclonal Antibody

Purification: Antigen Affinity

Immunogène: Recombinant EBOV (Subtype Sudan, strain Gulu) Glycoprotein / GP Protein (aa:Met1-Asn637, His Tag), ABIN7198907

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-1:5000

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 0.2 μm filtered solution in PBS

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze / thaw cycles.

Détails sur SEBOV GP

Antigène: SEBOV GP (Sudan Ebola Virus Envelope Glycoprotein (SEBOV GP))

Autre désignation: SEBOV Glycoprotein/GP (SEBOV GP Produits)

Sujet: Glycoprotein,GP,The fourth gene of the EBOV genome encodes a 16- kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.