ETF1 anticorps (C-Term)

N° du produit ABIN7466955

Détail du produit anti-ETF1 anticorps

Antigène: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ETF1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogène: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human eRF1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.91 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur ETF1

Antigène: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Autre désignation: eukaryotic translation termination factor 1 (ETF1 Produits)

Synonymes: anticorps D5S1995, anticorps ERF, anticorps ERF1, anticorps RF1, anticorps SUP45L1, anticorps TB3-1, anticorps AI463371, anticorps D6Ertd109e, anticorps eRF1, anticorps zeh0057, anticorps hm:zeh0057, anticorps wu:fa19e07, anticorps wu:fi31f01, anticorps cl1, anticorps RRF1, anticorps DDBDRAFT_0188023, anticorps DDBDRAFT_0191343, anticorps DDB_0188023, anticorps DDB_0191343, anticorps CG5605, anticorps Dm0184, anticorps Dmel\\CG5605, anticorps Group J, anticorps group J, anticorps l(3)00103, anticorps l(3)05637, anticorps l(3)77ABb, anticorps l(3)neo28, anticorps Eukaryotic release factor 1, anticorps eukaryotic translation termination factor 1, anticorps eukaryotic translation termination factor 1b, anticorps eukaryotic translation termination factor 1 S homeolog, anticorps eukaryotic peptide chain release factor subunit 1, anticorps eukaryotic petide chain release factor subunit 1, anticorps eukaryotic release factor 1, anticorps Eukaryotic peptide chain release factor subunit 1, anticorps ETF1, anticorps Etf1, anticorps etf1b, anticorps etf1, anticorps etf1.S, anticorps Tb11.22.0012, anticorps THAPSDRAFT_22240, anticorps TTHERM_00292170, anticorps LOC692937, anticorps erf1, anticorps eRF1, anticorps erfa-1

Sujet: Eukaryotic translation termination factor 1 , D5S1995 , ERF , ERF1 , RF1 , SUP45L1 , TB3-1,Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]

Poids moléculaire: 49 kDa

ID gène: 2107

UniProt: P62495