Recombinant CD22 anticorps (AA 52-178)
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- Antigène Voir toutes CD22 Anticorps
- CD22 (CD22 Molecule (CD22))
- Type d'anticorp
- Recombinant Antibody
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Épitope
- AA 52-178
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD22 est non-conjugé
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Application
- Immunohistochemistry (IHC)
- Immunogène
- Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)
- Clone
- MSVA-022R
- Isotype
- IgG
- Top Product
- Discover our top product CD22 Anticorps primaire
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- Indications d'application
- IHC 1:100-1:200
- Commentaires
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Positive Control: Tonsil or appendix: A strong, predominantly membranous CD22 staining of the germinal centre and mantle zone B-cells as well as of few interfollicular B-cells should be seen.
Negative Control: Tonsil or appendix: A large fraction of the interfollicular lymphocytes and all epithelial cells must not show any CD22 staining.
- Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Antigène
- CD22 (CD22 Molecule (CD22))
- Autre désignation
- CD22 (CD22 Produits)
- Synonymes
- anticorps A530093D23, anticorps Lyb-8, anticorps Lyb8, anticorps SIGLEC-2, anticorps SIGLEC2, anticorps siglec-2, anticorps CD22 antigen, anticorps CD22 molecule, anticorps Cd22, anticorps CD22
- Sujet
- B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14,CD22 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P20273
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