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Recombinant CD22 anticorps (AA 52-178)

CD22 Reactivité: Humain IHC Hôte: Lapin Monoclonal MSVA-022R unconjugated Recombinant Antibody
N° du produit ABIN7477403
  • Antigène Voir toutes CD22 Anticorps
    CD22 (CD22 Molecule (CD22))
    Type d'anticorp
    Recombinant Antibody
    Épitope
    • 15
    • 11
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 52-178
    Reactivité
    • 143
    • 52
    • 24
    • 5
    • 3
    • 2
    • 2
    • 1
    Humain
    Hôte
    • 91
    • 61
    • 16
    Lapin
    Clonalité
    • 115
    • 55
    Monoclonal
    Conjugué
    • 83
    • 15
    • 13
    • 11
    • 8
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CD22 est non-conjugé
    Application
    • 103
    • 73
    • 45
    • 38
    • 35
    • 20
    • 13
    • 12
    • 11
    • 8
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC)
    Immunogène
    Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)
    Clone
    MSVA-022R
    Isotype
    IgG
    Top Product
    Discover our top product CD22 Anticorps primaire
  • Indications d'application
    IHC 1:100-1:200
    Commentaires

    Positive Control: Tonsil or appendix: A strong, predominantly membranous CD22 staining of the germinal centre and mantle zone B-cells as well as of few interfollicular B-cells should be seen.

    Negative Control: Tonsil or appendix: A large fraction of the interfollicular lymphocytes and all epithelial cells must not show any CD22 staining.

    Protocole
    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Stock
    4 °C,-20 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • Antigène
    CD22 (CD22 Molecule (CD22))
    Autre désignation
    CD22 (CD22 Produits)
    Synonymes
    anticorps A530093D23, anticorps Lyb-8, anticorps Lyb8, anticorps SIGLEC-2, anticorps SIGLEC2, anticorps siglec-2, anticorps CD22 antigen, anticorps CD22 molecule, anticorps Cd22, anticorps CD22
    Sujet
    B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14,CD22 antibody validated for immunohistochemistry on 76 different Normal Tissues
    UniProt
    P20273
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