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CD27 anticorps (AA 28-170)

Name: CD27 anticorps (AA 28-170)
Brand: antibodies-online
SKU: ABIN7477407
Price: 1253.88 EUR
Availability: InStock

CD27 Reactivité: Humain IHC Hôte: Souris Monoclonal MSVA-027M unconjugated

tonsil surface epithelium (CD27 anticorps (AA 28-170))

IHC

Duodenum mucosa (CD27 anticorps (AA 28-170))

IHC

IHC

3 images

N° du produit ABIN7477407

Fiche de données

Antigène Voir toutes CD27 Anticorps

CD27 (CD27 Molecule (CD27))
Épitope
16

15

8

8

6

6

5

4

4

3

3

2

2

1

1

1

1

1

1

1

1

1

1
AA 28-170
Reactivité
192

76

53

9

5

5

3

2

1

1

1
Humain
Hôte
110

89

23

11

4

1

1
Souris
Anticorps secondaires appropriés
Clonalité
145

92

2
Monoclonal
Conjugué
83

24

23

15

13

6

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
Cet anticorp CD27 est non-conjugé
Application
156

78

50

37

35

24

17

14

13

13

10

9

6

5

4

3

3

2

2

2

2

2

1

1

1

1

1
Immunohistochemistry (IHC)

Immunogène

Recombinant human CD27 protein fragment (aa 28-170) (exact sequence is proprietary)

Clone

MSVA-027M

Isotype

IgG

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Indications d'application

IHC 1:100-1:200

Commentaires

Positive Control: Tonsil: A strong, membranous staining should be seen in the majority of interfollicular lymphocytes but also in a fraction of lymphocytes in the germinal centres and the mantle zone.
Negative Control: Tonsil: CD27 staining should be absent in all epithelial cells and a fraction of lymphocytes.

Protocole

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions

For Research Use only
Format

Liquid

Stock

4 °C,-20 °C,-80 °C

Stockage commentaire

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
Antigène

CD27 (CD27 Molecule (CD27))

Autre désignation

CD27 (CD27 Produits)

Sujet

LPFS2, S152, T cell activation antigen S152, T-cell activation antigen CD27, T14, TNFRSF7, TNFSF7, Tp55, Tumor necrosis factor receptor superfamily member 7,CD27 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt

P26842

Pathways

Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints