CD27 anticorps (AA 28-170)
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- Antigène Voir toutes CD27 Anticorps
- CD27 (CD27 Molecule (CD27))
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Épitope
- AA 28-170
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD27 est non-conjugé
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Application
- Immunohistochemistry (IHC)
- Immunogène
- Recombinant human CD27 protein fragment (aa 28-170) (exact sequence is proprietary)
- Clone
- MSVA-027M
- Isotype
- IgG
- Top Product
- Discover our top product CD27 Anticorps primaire
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- Indications d'application
- IHC 1:100-1:200
- Commentaires
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Positive Control: Tonsil: A strong, membranous staining should be seen in the majority of interfollicular lymphocytes but also in a fraction of lymphocytes in the germinal centres and the mantle zone.
Negative Control: Tonsil: CD27 staining should be absent in all epithelial cells and a fraction of lymphocytes.
- Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Antigène
- CD27 (CD27 Molecule (CD27))
- Autre désignation
- CD27 (CD27 Produits)
- Sujet
- LPFS2, S152, T cell activation antigen S152, T-cell activation antigen CD27, T14, TNFRSF7, TNFSF7, Tp55, Tumor necrosis factor receptor superfamily member 7,CD27 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P26842
- Pathways
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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