CD163 anticorps (AA 43-196)
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- Antigène Voir toutes CD163 Anticorps
- CD163
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Épitope
- AA 43-196
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD163 est non-conjugé
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Application
- Immunohistochemistry (IHC)
- Immunogène
- Recombinant fragment of human CD163 protein (around aa 43-196) (exact sequence is proprietary)
- Clone
- MSVA-163M
- Isotype
- IgG
- Top Product
- Discover our top product CD163 Anticorps primaire
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- Indications d'application
- IHC 1:100-1:200
- Commentaires
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Positive Control: A moderate to strong cytoplasmic staining of macrophages should be seen in the interfollicular zones of the tonsil, lamina propria of the appendix and in the Kupffer cells of the liver (see images). Macrophages surrounding the vessels in brain specimens should stain positive, while microglial cells should not show CD163 staining.
Negative Control: CD163 staining should not be seen in hepatocytes and in epithelial cells of the appendix and tonsil.
- Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Antigène
- CD163
- Autre désignation
- CD163 (CD163 Produits)
- Synonymes
- anticorps DMBT1, anticorps CD163, anticorps CD163v2, anticorps CD163v3, anticorps M130, anticorps MM130, anticorps deleted in malignant brain tumors 1 protein-like, anticorps CD163 molecule, anticorps CD163 antigen, anticorps LOC426820, anticorps CD163, anticorps Cd163
- Sujet
- CD163, CD163 antigen, Macrophage-associated antigen, M130, CD163 Molecule, Hemoglobin scavenger receptor, MM130, Scavenger receptor cysteine rich type 1 protein M130,CD163 antibody validated for immunohistochemistry on 76 different Normal Tissues
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