Recombinant Collagen IV anticorps
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- Antigène Voir toutes Collagen IV (COL4) Anticorps
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- Type d'anticorp
- Recombinant Antibody
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Collagen IV est non-conjugé
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Application
- Immunohistochemistry (IHC)
- Immunogène
- Purified human placental extract
- Clone
- MSVA-704R
- Isotype
- IgG
- Top Product
- Discover our top product COL4 Anticorps primaire
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- Indications d'application
- IHC 1:100-1:200
- Commentaires
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Positive Control: Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen.
Negative Control: Colon: Collagen IV immunostaining must be absent in epithelial cells.
- Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Antigène
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- Autre désignation
- Collagen IV (COL4 Produits)
- Synonymes
- anticorps collagen, type IV, anticorps Let-2
- Sujet
- Arresten, BSVD, COL4A1, COL4A1 NC1 domain, COL4A2, COL4A3, COL4A4, COL4A5, collagen alpha-1(IV) chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, Collagen Type IV Alpha 3, Collagen Type IV Alpha 4, Collagen Type IV Alpha 5, RATOR,Collagen IV antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P02462, P08572, P29400, P53420
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