Recombinant Nucleolin anticorps
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- Antigène Voir toutes Nucleolin (NCL) Anticorps
- Nucleolin (NCL)
- Type d'anticorp
- Recombinant Antibody
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Nucleolin est non-conjugé
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Application
- Immunohistochemistry (IHC)
- Immunogène
- Recombinant full-length human NCL protein
- Clone
- MSVA-623R
- Isotype
- IgG
- Top Product
- Discover our top product NCL Anticorps primaire
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- Indications d'application
- IHC 1:100-1:200
- Commentaires
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Positive Control: Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible - at least - in the superficial epithelial cell layer.
Negative Control: Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells.
- Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Antigène
- Nucleolin (NCL)
- Autre désignation
- Nucleolin (NCL Produits)
- Sujet
- NCL, Nucl, Nucleolin, Protein C23,Nucleolin antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P19338
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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