Recombinant Nucleolin anticorps

N° du produit ABIN7477541

Détail du produit anti-Nucleolin anticorps

Antigène: Nucleolin (NCL)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Nucleolin est non-conjugé

Application: Immunohistochemistry (IHC)

Immunogène: Recombinant full-length human NCL protein

Clone: MSVA-623R

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible - at least - in the superficial epithelial cell layer.

Negative Control: Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur Nucleolin

Antigène: Nucleolin (NCL)

Autre désignation: Nucleolin (NCL Produits)

Sujet: NCL, Nucl, Nucleolin, Protein C23,Nucleolin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization