Occludin anticorps (AA 282-415)
-
- Antigène Voir toutes Occludin (OCLN) Anticorps
- Occludin (OCLN)
-
Épitope
- AA 282-415
-
Reactivité
- Humain
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp Occludin est non-conjugé
-
Application
- Immunohistochemistry (IHC)
- Immunogène
- ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)
- Clone
- MSVA-415M
- Isotype
- IgG
- Top Product
- Discover our top product OCLN Anticorps primaire
-
-
- Indications d'application
- IHC 1:100-1:200
- Commentaires
-
Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.
Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.
- Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
- Antigène
- Occludin (OCLN)
- Autre désignation
- Occludin (OCLN Produits)
- Synonymes
- anticorps AI503564, anticorps Ocl, anticorps ocln, anticorps oclnb, anticorps tpmt, anticorps OCLN, anticorps wu:fd23h10, anticorps wu:fi13c01, anticorps zgc:113992, anticorps zgc:56359, anticorps BLCPMG, anticorps occludin, anticorps occludin S homeolog, anticorps occludin a, anticorps Ocln, anticorps OCLN, anticorps ocln.S, anticorps ocln, anticorps oclna
- Sujet
- BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PPP1R115, PTORCH1, Tight junction protein occludin,Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- Q16625
- Pathways
- Cell-Cell Junction Organization, Hepatitis C
-