Recombinant Melanoma gp100 anticorps
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- Antigène Voir toutes Melanoma gp100 (PMEL) Anticorps
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
- Type d'anticorp
- Recombinant Antibody
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Melanoma gp100 est non-conjugé
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Application
- Immunohistochemistry (IHC)
- Clone
- MSVA-617R
- Isotype
- IgG
- Top Product
- Discover our top product PMEL Anticorps primaire
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- Indications d'application
- IHC 1:100-1:200
- Commentaires
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Positive Control: Skin: A moderate to strong cytoplasmic PMEL immunostaining should be seen in all melanocytes of the skin.
Negative Control: Colon: PMEL immunostaining should be completely absent in all cells.
- Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Antigène
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
- Autre désignation
- PMEL (PMEL Produits)
- Sujet
- 95 kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog,PMEL / gp100 / Melanosome antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P40967
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