Recombinant Villin 1 anticorps (AA 600-700)
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- Antigène Voir toutes Villin 1 (VIL1) Anticorps
- Villin 1 (VIL1)
- Type d'anticorp
- Recombinant Antibody
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Épitope
- AA 600-700
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Villin 1 est non-conjugé
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Application
- Immunohistochemistry (IHC)
- Immunogène
- Recombinant fragment (around aa600-700) of human Villin protein (exact sequence is proprietary)
- Clone
- MSVA-459R
- Isotype
- IgG
- Top Product
- Discover our top product VIL1 Anticorps primaire
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- Indications d'application
- IHC 1:100-1:200
- Commentaires
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Positive Control: Colon: A moderate to strong villin staining with predominance of the apical membrane should be seen in all epithelial cells. Liver: A weak to moderate staining of the apical pole of hepatocytes should be seen.
Negative Control: Colon: Villin staining should be absent in all non-epithelial cells.
- Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Antigène
- Villin 1 (VIL1)
- Autre désignation
- Villin (VIL1 Produits)
- Synonymes
- anticorps D2S1471, anticorps VIL, anticorps Vil, anticorps vil1, anticorps MGC64339, anticorps VIL1, anticorps vil1l, anticorps zgc:55779, anticorps villin 1, anticorps villin 1 S homeolog, anticorps VIL1, anticorps Vil1, anticorps vil1.S, anticorps vil1
- Sujet
- VIL1, Villin-1, Villin1,Villin antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P09327
- Pathways
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
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