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Recombinant Villin 1 anticorps (AA 600-700)

VIL1 Reactivité: Humain IHC Hôte: Lapin Monoclonal MSVA-459R unconjugated Recombinant Antibody
N° du produit ABIN7477617
  • Antigène Voir toutes Villin 1 (VIL1) Anticorps
    Villin 1 (VIL1)
    Type d'anticorp
    Recombinant Antibody
    Épitope
    • 16
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 600-700
    Reactivité
    • 74
    • 25
    • 19
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 46
    • 28
    Lapin
    Clonalité
    • 38
    • 36
    Monoclonal
    Conjugué
    • 46
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Villin 1 est non-conjugé
    Application
    • 45
    • 27
    • 17
    • 17
    • 12
    • 8
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC)
    Immunogène
    Recombinant fragment (around aa600-700) of human Villin protein (exact sequence is proprietary)
    Clone
    MSVA-459R
    Isotype
    IgG
    Top Product
    Discover our top product VIL1 Anticorps primaire
  • Indications d'application
    IHC 1:100-1:200
    Commentaires

    Positive Control: Colon: A moderate to strong villin staining with predominance of the apical membrane should be seen in all epithelial cells. Liver: A weak to moderate staining of the apical pole of hepatocytes should be seen.

    Negative Control: Colon: Villin staining should be absent in all non-epithelial cells.

    Protocole
    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Stock
    4 °C,-20 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • Antigène
    Villin 1 (VIL1)
    Autre désignation
    Villin (VIL1 Produits)
    Synonymes
    anticorps D2S1471, anticorps VIL, anticorps Vil, anticorps vil1, anticorps MGC64339, anticorps VIL1, anticorps vil1l, anticorps zgc:55779, anticorps villin 1, anticorps villin 1 S homeolog, anticorps VIL1, anticorps Vil1, anticorps vil1.S, anticorps vil1
    Sujet
    VIL1, Villin-1, Villin1,Villin antibody validated for immunohistochemistry on 76 different Normal Tissues
    UniProt
    P09327
    Pathways
    EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
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