E. Coli anticorps (FITC)

N° du produit ABIN7478177

Détail du produit anti-E. Coli anticorps (FITC)

Antigène: E. Coli

Reactivité: E. coli

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp E. Coli est conjugé à/à la FITC

Application: Functional Studies (Func)

Specificité: Many "O" and "K" antigenic serotypes of Escherichia coli

Réactivité croisée (Details): Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae

Purification: This product consists of purified IgG fraction of the neat antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Immunogène: Mixture of E. coli serotypes

Isotype: IgG

Information d'application

Indications d'application: TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.

Détails sur E. Coli

Antigène: E. Coli

Abstract: E. Coli Produits

Classe de substances: Bacteria