E. Coli anticorps (Peroxidase (POD))
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- Antigène Tous les produits E. Coli
- E. Coli
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Reactivité
- E. coli
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp E. Coli est conjugé à/à la Peroxidase (POD)
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (IHC)
- Specificité
- Many "O" and "K" antigenic serotypes of Escherichia coli
- Réactivité croisée (Details)
- Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae
- Purification
- This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
- Immunogène
- Mixture of E. coli serotypes
- Isotype
- IgG
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- Indications d'application
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TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
- Agent conservateur
- Thimerosal (Merthiolate)
- Précaution d'utilisation
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
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- Antigène
- E. Coli
- Abstract
- E. Coli Produits
- Classe de substances
- Bacteria
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