ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
Specificité
Numerous Adeno proteins including hexon
Réactivité croisée (Details)
Reactive with Adeno types 1, 2, 3, 5, 6, 7a, 8, 31, 40 & 41. Others not tested. Does not crossreact with Para 1-3, Infl. A & B, RSV, hMPV & Rhinovirus (type 16) UNINFECTED CELL REACTIVITY: Negative vs. HEp-2, LLCMK2 & Vero cells by indirect immunofluorescence
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
Immunogène
gradient purified, disrupted Adenovirus type 5 virions
TITER : > 1: 5,000 by ELISA, > 1:8,000 in IFA, > 1:1,000 in western blot immunoassay, Possible applications for this product include avidin and streptavidin amplification systems for immunohistochemistry, ELISA, fluorescence microscopy and immunoblotting. In addition, this product may be used in place of neat antiserum in almost any appropriate antibody-based technique.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1 % sodium azide preservative. No stabilizing proteins have been added.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.