Cet anticorp Canine Heartworm est conjugé à/à la Peroxidase (POD)
Application
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (IHC)
Specificité
Soluble & structural antigens of the adult worm
Réactivité croisée (Details)
Antiserum is not absorbed for related filarial Nematodes UNINFECTED CELL REACTIVITY: Non-reactive with canine serum
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
TITER : > 1:6,000 by ELISA, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.