Cet anticorp Mycoplasma Pneumoniae est conjugé à/à la Peroxidase (POD)
Application
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (IHC)
Specificité
All antigens
Réactivité croisée (Details)
This antiserum has not been absorbed and may react with related microorganisms UNINFECTED CELL REACTIVITY: Not reactive with bovine or human sera
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
TITER : >1:1,000 by indirect IFA, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.