Dysferlin anticorps (AA 1999-2016)

N° du produit ABIN7538896

Détail du produit anti-Dysferlin anticorps

Antigène: Dysferlin (DYSF)

Épitope: AA 1999-2016

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Dysferlin est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Fonction: Lyophilized Mouse Monoclonal Antibody Dysferlin

Specificité: Reactive with the dysferlin molecule in human skeletal muscle. Also present in many non-muscle tissues.

Attributs du produit: This product is intended for the qualitative identification by light microscopy of Dysferlin by immunohistochemistry.

Purification: Tissue culture supernatant

Immunogène: Synthetic peptide containing amino acids 1999-2016 of the human dysferlin molecule.

Clone: Ham1-7B6

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on frozen sections: Suggested dilution: 1:20-1:40 for 60 minutes at 25°C. This is provided as a guide and users should determine their own optimal working dilutions. NCL-Hamlet requires the sections to be fixed in acetone/ methanol at a ratio of 1:1 for 4 minutes at 25°C prior to incubation with the primary antibody. Immunohistochemistry on paraffin sections. Suggested dilution: 1:20-1:40 for 60 minutes at 25°C. High temperature antigen retrieval using 0.01 M citrate retrieval solution ( pH 6.0) is recommended. This is provided as a guide and users should determine their own optimal working dilutions.,Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.

Concentration: 180 mg/L

Buffer: This product is a lyophilized tissue culture supernatant containing sodium azide as a preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Date de péremption: 12 months

Détails sur Dysferlin

Antigène: Dysferlin (DYSF)

Autre désignation: Dysferlin (DYSF Produits)

Synonymes: anticorps DYSF, anticorps fb73b05, anticorps wu:fb73b05, anticorps si:rp71-50c18.1, anticorps DKFZp459E1226, anticorps 2310004N10Rik, anticorps AI604795, anticorps D6Pas3, anticorps mFLJ00175, anticorps FER1L1, anticorps LGMD2B, anticorps MMD1, anticorps dysferlin, anticorps dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive), anticorps myoferlin, anticorps DYSF, anticorps dysf, anticorps LOC589501, anticorps Dysf