This antibody is expressed in HEK293 cells and and was purified using a combination of Protein A/G chromatography. It is reactive against the EV membrane protein of monkeypox virus (Homologous to Vaccinia virus B5R). It also reacts to VACV antigen and lysate, CPXV lysate, MPXV lysate, and VARV antigen and lysate. MPXV-13 weakly binds to VACV purified antigen, VACV and MPXV virus-infected cell lysates, and strongly binds VARV purified antigen and CPXV virus-infected cell lysate. This product is sold for research use only. Optimal working concentrations should be determined by each researcher.
Purification
This human recombinant IgG1 antibody is purified using a multiple step process to include Protein A or Protein G chromatography. It is produced using in vitro cell culture techniques. Antibody concentration was determined using an absorbance at 280 nm: 1.4 equals 1.0 mg of IgG.
Pureté
Purified Protein
Clone
1E2
Isotype
IgG1
Indications d'application
This antibody reacts with monkeypox virus as determined by ELISA. Optimal working dilutions should be determined experimentally by the investigator.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS, pH 7.2, 0.09 % Sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-80 °C
Stockage commentaire
Antibody may be stored between 2°C and 8°C for up to 30 days. For long-term storage, aliquot and store at -80°C. Multiple freeze-thaw cycles are not recommended.