DNAJB9 anticorps

N° du produit ABIN7539265

Détail du produit anti-DNAJB9 anticorps

Antigène: DNAJB9 (Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNAJB9 est non-conjugé

Application: Western Blotting (WB)

Fonction: Mdg-1 antibody

Purification: Protein-A purified

Immunogène: Recombinant human Mdg-1

Information d'application

Indications d'application: Western Blot: Use 1-5 μg/mL

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Date de péremption: 24 months

Détails sur DNAJB9

Antigène: DNAJB9 (Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9))

Autre désignation: Mdg-1 (DNAJB9 Produits)

Synonymes: anticorps ERdj4, anticorps MDG-1, anticorps MDG1, anticorps MST049, anticorps MSTP049, anticorps AA408011, anticorps AA673251, anticorps AA673481, anticorps AW556981, anticorps Mdg1, anticorps mDj7, anticorps DnaJ heat shock protein family (Hsp40) member B9, anticorps DNAJB9, anticorps Dnajb9

Sujet: Microvascular endothelial differentiation gene 1 protein, DnaJ homolog subfamily B member 9, ERdj4,Angiogenesis research has focused on receptors and ligands mediating endothelial cell proliferation and migration. Little is known about the molecular mechanisms that are involved in converting endothelial cells from a proliferative to a differentiated state. Microvascular differentiation gene 1 (Mdg1) has been isolated from differentiating microvascular endothelial cells that had been cultured in collagen type I gels (3D culture). In adult human tissue Mdg1 is expressed in endothelial and epithelial cells. Sequence analysis of the full-length cDNA revealed that the N-terminal region of the putative Mdg1-protein exhibits a high sequence similarity to the J-domain of Hsp40 chaperones. It was shown that this region functions as a bona fide J-domain as it can replace the J-domain of Escherichia coli DnaJ-protein. Mdg1 is also upregulated in primary endothelial and mesangial cells when subjected to various stress stimuli. GFP-Mdg1 fusion constructs showed the Mdg1-protein to be localized within the cytoplasm under control conditions. Stress induces the translocation of Mdg1 into the nucleus, where it accumulates in nucleoli. Costaining with Hdj1, Hdj2, Hsp70, and Hsc70 revealed that Mdg1 colocalizes with Hsp70 and Hdj1 in control and stressed HeLa cells. These data suggest that Mdg1 is involved in the control of cell cycle arrest taking place during terminal cell differentiation and under stress conditions.

ID gène: 4189

NCBI Accession: NM_012328, NP_036460

UniProt: Q9UBS3

Pathways: ER-Nucleus Signaling