Nuclease anticorps

N° du produit ABIN7540666

N° du produit (Fournisseur): 200-401-mu6

Détail du produit anti-Nuclease anticorps

Antigène: Nuclease

Reactivité: Serratia marcescens

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nuclease est non-conjugé

Application: ELISA, Western Blotting (WB)

N° du produit (Fournisseur): 200-401-mu6

Fournisseur: Rockland

Fonction: Nuclease/NucA Antibody

Réactivité croisée (Details): This antibody will react with endonuclease from S.

Purification: This antibody was purified from monospecific antiserum by Protein A chromatography.

Stérilité: Sterile filtered

Immunogène: Anti-Nuclease/NucA was prepared by repeated immunizations with recombinant benzonase protein produced in E. coli.

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:21,600 - 1:22,600

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-Nuclease/NucA has been tested by ELISA and western blot. Expect a band ~ 28 kDa in size corresponding to endonuclease by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Nuclease

Antigène: Nuclease

Autre désignation: nucA (Nuclease Produits)

Sujet: Benzonase, Nuclease, Endonuclease, Serratia marcescens endonuclease, NucA,Nucleases are secreted by Serratia marcescens into the medium it surrounds. The enzyme is a sugar-nonspecific hydrolase, capable of cleaving both RNA and DNA in either double or single stranded form. It requires divalent cations, preferably Mg2+, and is functional across a broad pH range from 6 to 10 (optimal at 8-8.5) and wide temperature ranges between 35 °C and 44 °C. The ability of Serratia to secrete nuclease appears to be regulated. Bacterial cultures at differing cell densities display different kinetics and efficiencies of nuclease secretion [i.e. growth medium, growth conditions, and host cell mutations]. Anti-Nuclease/NucA Antibody is useful for researcher interested in identifying nucleic acid contamination.

UniProt: P13717