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Leptin anticorps (AA 101-154)

Name: Leptin anticorps (AA 101-154)
SKU: ABIN725030
Price: 384.62 EUR
Availability: InStock

LEP Reactivité: Souris, Rat ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Formalin-fixed and paraffin embedded: rat liver labeled with Anti-Leptin Polyclonal Antibody (ABIN725030), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining (Leptin anticorps (AA 101-154))

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Leptin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Leptin anticorps (AA 101-154))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse liver, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Leptin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Leptin anticorps (AA 101-154))

IHC (p)

3 images

N° du produit ABIN725030

Fiche de données

Antigène Voir toutes Leptin (LEP) Anticorps

Leptin (LEP)
Épitope
25

15

15

15

12

12

7

5

4

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 101-154
Reactivité
129

75

72

12

8

7

5

3

3

3

2

1

1

1
Souris, Rat
Hôte
171

30

7

5

1
Lapin
Anticorps secondaires appropriés
Clonalité
182

31
Polyclonal
Conjugué
104

24

16

8

8

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

1

1

1

1

1

1

1
Cet anticorp Leptin est non-conjugé
Application
129

82

62

38

38

37

32

30

22

18

15

11

10

7

5

4

2

2

2

1

1

1

1

1

1

1
ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée

Souris, Rat

Homologie

Dog,Cow,Pig,Horse

Purification

Purified by Protein A.

Immunogène

KLH conjugated synthetic peptide derived from mouse Leptin

Isotype

IgG

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LEP Reactivité: Humain WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

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LEP Reactivité: Rat WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

anti-Leptin (LEP) (AA 17-100) antibody
LEP Reactivité: Humain, Souris, Rat WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Leptin (LEP) (AA 8-37) antibody
LEP Reactivité: Humain WB, ELISA, IHC, IF, FACS Hôte: Lapin Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivité: Souris WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

anti-Leptin (LEP) (AA 29-160) antibody
LEP Reactivité: Boeuf (Vache) WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivité: Humain WB, IF Hôte: Lapin Polyclonal unconjugated

anti-Leptin (LEP) (AA 7-37), (N-Term) antibody
LEP Reactivité: Humain WB, IF, FACS, EIA, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivité: Humain WB, IHC, IP, ICC Hôte: Souris Monoclonal C5 unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivité: Chien WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

Indications d'application

ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption

12 months
Antigène

Leptin (LEP)

Autre désignation

Leptin (LEP Produits)

Synonymes

anticorps ob, anticorps obese, anticorps LEPD, anticorps OB, anticorps OBS, anticorps leptin, anticorps Lep, anticorps LEP, anticorps lep

Sujet

Synonyms: ob, obese, Leptin, Obesity factor, Lep
Background: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

ID gène

16846

UniProt

P41160

Pathways

Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process