Pour une meilleure utilisation, nous vous conseillons d’activer Javascript dans votre navigateur.

PUMA anticorps (AA 131-180)

Name: PUMA anticorps (AA 131-180)
SKU: ABIN731528
Price: 384.62 EUR
Availability: InStock

BBC3 Reactivité: Humain, Rat, Porc WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Erianin induced mitochondrial apoptosis in liver cancer cells. (PUMA anticorps (AA 131-180))

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BBC3/PUMA Polyclonal Antibody, Unconjugated (ABIN731528) at 1:200 followed by conjugation to the secondary antibody and DAB staining (PUMA anticorps (AA 131-180))

IHC

K562 lysates probed with PUMA Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. (PUMA anticorps (AA 131-180))

U87MG cells probed with Puma Polyclonal Antibody, Unconjugated at 1:50 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (PUMA anticorps (AA 131-180))

FACS

4 images

(3 références)

N° du produit ABIN731528

Fiche de données

Antigène Voir toutes PUMA (BBC3) Anticorps

PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
Épitope
15

13

9

8

7

6

6

6

5

5

4

2

1

1

1

1

1

1

1

1
AA 131-180
Reactivité
91

39

38

15
Humain, Rat, Porc
Hôte
88

6

1
Lapin
Anticorps secondaires appropriés
Clonalité
80

15
Polyclonal
Conjugué
47

6

5

5

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp PUMA est non-conjugé
Application
76

33

29

20

15

13

12

12

8

7

5

2

2

2
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée

Humain, Porc, Rat

Homologie

Mouse,Dog,Cow

Purification

Purified by Protein A.

Immunogène

KLH conjugated synthetic peptide derived from human PUMA

Isotype

IgG

Top Product

Discover our top product BBC3 Anticorps primaire
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody
BBC3 Reactivité: Humain WB, ICC, IF Hôte: Lapin Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibody
BBC3 Reactivité: Humain WB, FACS, IHC (p), IF Hôte: Lapin Polyclonal RB01353 unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibody
BBC3 Reactivité: Humain WB, ELISA, IHC, FACS, IF Hôte: Lapin Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody
BBC3 Reactivité: Humain WB, IHC Hôte: Lapin Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (PE)
BBC3 Reactivité: Humain WB, ICC, IF Hôte: Lapin Polyclonal PE

anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (PerCP)
BBC3 Reactivité: Humain WB, ICC, IF Hôte: Lapin Polyclonal PerCP

anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody (Biotin)
BBC3 Reactivité: Humain WB, IHC Hôte: Lapin Polyclonal Biotin

anti-BCL2 Binding Component 3 (BBC3) (BH3 Domain), (C-Term) antibody
BBC3 Reactivité: Humain, Souris WB, FACS, IHC (p), IF, EIA Hôte: Lapin Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 109-137) antibody
BBC3 Reactivité: Humain WB, IHC (p) Hôte: Lapin Polyclonal RB33714 unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 98-152) antibody
BBC3 Reactivité: Humain WB, ELISA Hôte: Souris Monoclonal 5D10 unconjugated

Indications d'application

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg)

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption

12 months
Su, Shi, Yan, Xi, Su: "Anticancer bioactive peptides suppress human colorectal tumor cell growth and induce apoptosis via modulating the PARP-p53-Mcl-1 signaling pathway." dans: Acta pharmacologica Sinica, Vol. 36, Issue 12, pp. 1514-9, (2016) (PubMed).

Qiu, Cai, Gao, Gu, Liu: "A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53." dans: Cancer letters, Vol. 356, Issue 2 Pt B, pp. 910-7, (2014) (PubMed).

Zhang, Liu, Xia, Zhang, Zhang, Zhao, Chao, Jiang, Jiang: "Epithelial-mesenchymal transition is necessary for acquired resistance to cisplatin and increases the metastatic potential of nasopharyngeal carcinoma cells." dans: International journal of molecular medicine, Vol. 33, Issue 1, pp. 151-9, (2013) (PubMed).
Antigène

PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Autre désignation

PUMA (BBC3 Produits)

Synonymes

anticorps BBC3, anticorps Puma, anticorps JFY-1, anticorps JFY1, anticorps PUMA, anticorps PUMA/JFY1, anticorps BCL2 binding component 3, anticorps Bcl-2 binding component 3, anticorps BBC3, anticorps Bbc3, anticorps bbc3

Sujet

Synonyms: JFY1, PUMA, JFY-1, Bcl-2-binding component 3, p53 up-regulated modulator of apoptosis, BBC3
Background: Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity.

ID gène

27113

UniProt

Q96PG8

Pathways

Signalisation p53, Positive Regulation of Endopeptidase Activity