DAP Kinase 1 anticorps (AA 1331-1430)

N° du produit ABIN732983

Détail du produit anti-DAP Kinase 1 anticorps

Antigène: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Épitope: AA 1331-1430

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DAP Kinase 1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Homologie: Human,Mouse,Rat

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human DAPK1

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-DAP Kinase 1 (ABIN732983)

Liu, Ao, Zhou, Cui, Zhou, Yuan, Xiang, Cao, Liu: "CpG island hypermethylation of multiple tumor suppressor genes associated with loss of their protein expression during rat lung carcinogenesis induced by 3-methylcholanthrene and diethylnitrosamine." dans: Biochemical and biophysical research communications, Vol. 402, Issue 3, pp. 507-14, (2010) (PubMed).

Détails sur DAP Kinase 1

Antigène: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Autre désignation: DAPK1 (DAPK1 Produits)

Synonymes: anticorps DAPK1, anticorps MGC81366, anticorps si:ch211-66i11.1, anticorps wu:fj09c03, anticorps dapk, anticorps MGC83745, anticorps 2310039H24Rik, anticorps 2810425C21Rik, anticorps AI642003, anticorps D13Ucla1, anticorps DAP-Kinase, anticorps DAPK, anticorps death-associated protein kinase 1, anticorps death associated protein kinase 1 L homeolog, anticorps death associated protein kinase 1, anticorps death associated protein kinase 1 S homeolog, anticorps LOC427465, anticorps dapk1.L, anticorps DAPK1, anticorps dapk1, anticorps dapk1.S, anticorps Dapk1

Sujet:

Synonyms: DAPK, Death-associated protein kinase 1, DAP kinase 1, DAPK1

Background: Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-133 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition. Isoform 2 cannot induce apoptosis but can induce membrane blebbing.

ID gène: 1612

UniProt: P53355

Pathways: Signalisation MAPK, Interferon-gamma Pathway