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GDNF anticorps (AA 121-211)

GDNF Reactivité: Humain, Souris, Rat, Boeuf (Vache), Lapin WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN736536
  • Antigène Voir toutes GDNF Anticorps
    GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
    Épitope
    • 15
    • 9
    • 8
    • 6
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 121-211
    Reactivité
    • 83
    • 41
    • 31
    • 15
    • 15
    • 1
    Humain, Souris, Rat, Boeuf (Vache), Lapin
    Hôte
    • 78
    • 6
    • 3
    • 3
    Lapin
    Clonalité
    • 76
    • 13
    • 1
    Polyclonal
    Conjugué
    • 39
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp GDNF est non-conjugé
    Application
    • 74
    • 28
    • 24
    • 18
    • 17
    • 12
    • 12
    • 12
    • 6
    • 6
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
     Réactivité croisée
    Boeuf (Vache), Humain, Souris, Lapin, Rat
    Homologie
    Dog,Cow,Pig,Horse,Chicken
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human GDNF
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #102003 (Immunofluorescence)
    'Independent Validation' signe
    by
    Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
    No.
    #102003
    Date
    12.02.2018
    Antigène
    GDNF
    Numéro du lot
    9F01M8
    Application validée
    Immunofluorescence
    Contrôle positif
    mouse hippocampus and putamen
    Contrôle négative
    no primary control
    Conclusion

    ABIN736536 works in IF albeit with some background staining that we have been unable to eliminate although using different dilutions to increase the signal-to-noise ratio.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN736536
    Anticorps secondaire
    anti-rabbit AF488 conjugated antibody (Life Technologies)
    Full Protocol
    • Perfuse mouse with 4% paraformaldehyde in 0.1M phosphate buffer (PB) pH7.4.
    • Post-fix spinal cord and brain blocks in the same fixative for additional 2h at RT.
    • Wash spinal cord and brain blocks several times with PBS.
    • Cut blocks with a vibratome (Leica, VT1000 S) into 70µm thick transverse sections.
    • Cut dorsal root ganglions (DRGs) with a cryostat into 17µm thick sections after cryoprotection and glass mounting.
    • Block free floating vibratome and glass mounted cryostat sections with blocking solution (0.01M PBS5% Normal Goat Serum (NGS; Sigma, G9023, lot SLBV1396), 0.1% Triton X-100 (BioRad, 161-0407, lot 00583) for 1h at RT.
    • Incubate sections with primary rabbit anti-GDNF antibody (antibodies-online, ABIN736536, lot 9F01M8) diluted 1:100 in blocking solution ON at RT.
    • Wash sections 4x for 5min with 0.01M PBS.
    • Incubate sections with secondary goat anti-rabbit AF488 conjugated antibody (Life Technologies) diluted 1:500 in PBS for 1h at RT.
    • Wash sections 4x for 5min with 0.01M PBS.
    • Mount sections in Fluoroshield (Sigma, F6182, lot MKCB0153V).
    Notes
    • Different dilutions were also tested (1:200, 1:500, 1:2000) with or without Triton-X but the 1:100 dilution and the use of Triton-X in the blocking solution gave the best results.

    • Staining is mainly present in the cytoplasm of small-size neurons in the DRG, consistent with what has been previously described. ABIN736536 stains fibers in superficial laminae of the dorsal horn of the spinal cord. In the hippocampus staining is present in the CA1 both in the pyramidal neurons (cell body and dendrites) and in individual interneurons. In the putamen ABIN736536 stains neuron cell bodies and some dendrites.

  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Zhang, Cai, Song, Dong, Hou, Lv: "Normalization of ventral tegmental area structure following acupuncture in a rat model of heroin relapse." dans: Neural regeneration research, Vol. 9, Issue 3, pp. 301-7, (2014) (PubMed).

  • Antigène
    GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
    Autre désignation
    GDNF (GDNF Produits)
    Synonymes
    anticorps gdnf, anticorps GDNF, anticorps AI385739, anticorps gdnfa, anticorps hfb1-gdnf, anticorps ATF1, anticorps ATF2, anticorps HFB1-GDNF, anticorps HSCR3, anticorps glial cell derived neurotrophic factor, anticorps glial cell derived neurotrophic factor a, anticorps glial cell line derived neurotrophic factor, anticorps glial cell derived neurotrophic factor L homeolog, anticorps Gdnf, anticorps gdnfa, anticorps GDNF, anticorps gdnf.L
    Sujet

    Synonyms: ATF1, ATF2, HSCR3, HFB1-GDNF, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF

    Background: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

    ID gène
    2668
    UniProt
    P39905
    Pathways
    Signalisation RTK, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
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