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Protein A anticorps

Reactivité: Staphylococcus aureus ELISA, WB, IF (p), IF (cc), IHC (fro), IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN739261
  • Antigène Voir toutes Protein A Anticorps
    Protein A
    Reactivité
    • 49
    • 5
    • 1
    Staphylococcus aureus
    Hôte
    • 19
    • 15
    • 14
    • 5
    • 2
    Lapin
    Clonalité
    • 48
    • 2
    Polyclonal
    Conjugué
    • 10
    • 8
    • 8
    • 7
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Protein A est non-conjugé
    Application
    • 35
    • 33
    • 12
    • 12
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
     Réactivité croisée
    Bactéries
    Réactivité croisée (Details)
    S. aureus
    Purification
    Purified by Protein A.
    Immunogène
    Recombinant protein A
    Isotype
    IgG
    Top Product
    Discover our top product Protein A Anticorps primaire
  • Indications d'application
    WB 1:100-1000
    IHC-P 1:100-500
    IF(IHC-P) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Antigène
    Protein A
    Abstract
    Protein A Produits
    Synonymes
    anticorps alpha protein, anticorps repB
    Sujet

    Synonyms: Immunoglobulin G binding protein A, Staphylococcal protein A, SPA, gG-binding protein A, Staphylococcus aureus.

    Background: Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.

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