H2AFX anticorps (pSer140)
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- Antigène Voir toutes H2AFX Anticorps
- H2AFX (H2A Histone Family, Member X (H2AFX))
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Épitope
- pSer140
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp H2AFX est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificité
- This phosphorylation site is homologous to that of Ser140 in Mouse and Rat.
- Réactivité croisée
- Humain
- Homologie
- Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit
- Purification
- Purified by Protein A.
- Immunogène
- KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140
- Isotype
- IgG
- Top Product
- Discover our top product H2AFX Anticorps primaire
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- Indications d'application
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Agent conservateur
- ProClin
- Précaution d'utilisation
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Date de péremption
- 12 months
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Reactive oxygen species induced by icaritin promote DNA strand breaks and apoptosis in human cervical cancer cells." dans: Oncology reports, Vol. 41, Issue 2, pp. 765-778, (2019) (PubMed).
: "Dihydroartemisinin induces autophagy-dependent death in human tongue squamous cell carcinoma cells through DNA double-strand break-mediated oxidative stress." dans: Oncotarget, Vol. 8, Issue 28, pp. 45981-45993, (2018) (PubMed).
: "Unpredicted Downregulation of RAD51 Suggests Genome Instability Induced by Tetrachlorobenzoquinone." dans: Chemical research in toxicology, Vol. 29, Issue 12, pp. 2184-2193, (2016) (PubMed).
: "Nutlin-3-induced redistribution of chromatin-bound IFI16 in human hepatocellular carcinoma cells in vitro is associated with p53 activation." dans: Acta pharmacologica Sinica, Vol. 36, Issue 2, pp. 252-8, (2015) (PubMed).
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Reactive oxygen species induced by icaritin promote DNA strand breaks and apoptosis in human cervical cancer cells." dans: Oncology reports, Vol. 41, Issue 2, pp. 765-778, (2019) (PubMed).
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- Antigène
- H2AFX (H2A Histone Family, Member X (H2AFX))
- Autre désignation
- Histone H2a.X (H2AFX Produits)
- Synonymes
- anticorps H2A.X, anticorps H2A/X, anticorps H2AX, anticorps AW228881, anticorps H2ax, anticorps Hist5-2ax, anticorps gammaH2ax, anticorps zgc:56329, anticorps h2a.x, anticorps h2a/x, anticorps h2ax, anticorps RGD1566119, anticorps h2a, anticorps h2afx, anticorps H2A histone family member X, anticorps H2A histone family, member X, anticorps H2A histone family member X L homeolog, anticorps histone cluster 1, H2ah, anticorps histone cluster 2, H2ab S homeolog, anticorps histone H2AX, anticorps H2AFX, anticorps H2afx, anticorps h2afx, anticorps h2afx.L, anticorps HIST1H2AH, anticorps hist2h2ab.S, anticorps LOC100522201, anticorps LOC100720536
- Sujet
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Synonyms: H2AX, H2A.X, H2A/X, Histone H2AX, Histone H2A.X, H2AFX
Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
- ID gène
- 3014
- UniProt
- P16104
- Pathways
- Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus
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