IKK-alpha /IKK-beta anticorps (pSer176, pSer180)

N° du produit ABIN743318

Détail du produit anti-IKK-alpha /IKK-beta anticorps

Antigène: IKK-alpha /IKK-beta

Épitope: pSer176, pSer180

Reactivité: Souris, Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IKK-alpha /IKK-beta est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: These phosphorylation sites are homologous across the listed species.

Réactivité croisée: Humain, Souris

Homologie: Rat,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human IKK alpha/beta around the phosphorylation site of Ser176/Ser180

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-IKK-alpha /IKK-beta (ABIN743318)

Zhao, Zheng, Hu, Shen, Li: "Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway." dans: Life sciences, Vol. 144, pp. 185-93, (2016) (PubMed).

Gao, Guo, Wang, Lin, Yu, Sheng, Wu, Gong: "Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells." dans: Acta histochemica, Vol. 118, Issue 6, pp. 606-14, (2016) (PubMed).

Détails sur IKK-alpha /IKK-beta

Antigène: IKK-alpha /IKK-beta

Autre désignation: IKK alpha/beta / (IKK-alpha /IKK-beta Produits)

Sujet:

Synonyms: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IkappaB kinase, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, Nuclear factor NF-kappa-B inhibitor kinase alpha, Transcription factor 16, TCF-16, CHUK

Background: Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.

ID gène: 1147

UniProt: O15111