Osteoactivin anticorps (AA 81-180)

N° du produit ABIN749093

Détail du produit anti-Osteoactivin anticorps

Antigène: Osteoactivin (GPNMB)

Épitope: AA 81-180

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Osteoactivin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Humain, Souris, Rat

Homologie: Cow,Pig,Horse,Guinea Pig

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human GPNMB

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Independent Validation (IHC (fp))

Validation #100002 (Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections))

by: Memorial Sloan-Kettering Cancer Center, Parada lab

No.: #100002

Date: 15.10.2015

Antigène: Osteoactivin

Application validée: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Contrôle positif: Tumor cell transplanted mouse brain was dissected

Conclusion: The staining pattern was as expected for Osteoactivin as membrane protein.

Validation Images

IF validation of osteoactivin antibody ABIN749093 on mouse brain tumor sections. A. ABIN749093 diluted 1:100 (red), DAPI counterstaining (blue). B. Dilution factor 1:50. C. Dilution factor 1:100. D. Dilution factor 1:200.

Protocole

Anticorps primaire: ABIN749093

Anticorps secondaire: Donkey-anti-Rabbit-Cy3

Full Protocol:

• Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
• Immunostaining for Cryo Sections:
• Dry slides at 50-55°C for 15-30 min (or RT for 30min)
• Wash three times with PBS 5min (shake gently)
• Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
• Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
• Rabbit-anti-Osteoactivin antibody (ABIN749093) diluted 1:50-1:400
• Wash three times with PBS 5min (shake gently)
• Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
• Donkey-anti-Rabbit-Cy3
• DAPI 1:100
• Wash three times with PBS 5min (shake gently)
• Add three drops of water-based Immu-mount
• Cover with 24x60 cover glass
• Imaging
• Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Notes: ABIN749093 worked within the diution range 1:50 - 1:200; the antibody did not work well at a 1:400 dilution.

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-Osteoactivin (ABIN749093)

Neal, Boyle, Budge, Safadi, Richardson: "The glycoprotein GPNMB attenuates astrocyte inflammatory responses through the CD44 receptor." dans: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 73, (2019) (PubMed).

Oyewumi, Manickavasagam, Novak, Wehrung, Paulic, Moussa, Sondag, Safadi: "Osteoactivin (GPNMB) ectodomain protein promotes growth and invasive behavior of human lung cancer cells." dans: Oncotarget, Vol. 7, Issue 12, pp. 13932-44, (2017) (PubMed).

Arosarena, Dela Cadena, Denny, Bryant, Barr, Thorpe, Safadi: "Osteoactivin Promotes Migration of Oral Squamous Cell Carcinomas." dans: Journal of cellular physiology, Vol. 231, Issue 8, pp. 1761-70, (2016) (PubMed).

Frara, Abdelmagid, Tytell, Amin, Popoff, Safadi, Barbe: "Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping." dans: BMC musculoskeletal disorders, Vol. 17, pp. 34, (2016) (PubMed).

Ruan, Li, Nie, Zhou, Zou: "Differential expression of glycoprotein non-metastatic melanoma protein B (GPNMB) involved in trichostatin A-induced apoptosis in gastric cancer." dans: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 4857-66, (2015) (PubMed).

Abdelmagid, Sondag, Moussa, Belcher, Yu, Stinnett, Novak, Mbimba, Khol, Hankenson, Malcuit, Safadi: "Mutation in Osteoactivin Promotes Receptor Activator of NF?B Ligand (RANKL)-mediated Osteoclast Differentiation and Survival but Inhibits Osteoclast Function." dans: The Journal of biological chemistry, Vol. 290, Issue 33, pp. 20128-46, (2015) (PubMed).

Détails sur Osteoactivin

Antigène: Osteoactivin (GPNMB)

Autre désignation: GPNMB (GPNMB Produits)

Synonymes: anticorps HGFIN, anticorps NMB, anticorps DC-HIL, anticorps Dchil, anticorps ipd, anticorps glycoprotein nmb, anticorps glycoprotein (transmembrane) nmb, anticorps glycoprotein nmb L homeolog, anticorps Gpnmb, anticorps GPNMB, anticorps gpnmb.L

Sujet:

Synonyms: NMB, HGFIN, Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN, GPNMB, UNQ1725/PRO9925

Background: The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

ID gène: 10457

UniProt: Q14956