SMURF2 anticorps (AA 601-700)

N° du produit ABIN753178

Détail du produit anti-SMURF2 anticorps

Antigène: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Épitope: AA 601-700

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMURF2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Humain, Souris, Rat

Homologie: Cow,Horse,Chicken

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human SMURF2

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-SMURF2 (ABIN753178)

Huang, Yang, Nan, Gao, Feng, Gou, Chen, Zhang, Yan, Peng, Xu: "The proteasome inhibitor, MG132, attenuates diabetic nephropathy by inhibiting SnoN degradation in vivo and in vitro." dans: BioMed research international, Vol. 2014, pp. 684765, (2015) (PubMed).

Détails sur SMURF2

Antigène: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Autre désignation: SMURF2 (SMURF2 Produits)

Synonymes: anticorps SMURF2, anticorps 2810411E22Rik, anticorps AI558114, anticorps AI649275, anticorps RGD1310067, anticorps wu:fb82a11, anticorps wu:fc41c02, anticorps SMAD specific E3 ubiquitin protein ligase 2, anticorps SMAD specific E3 ubiquitin protein ligase 2 S homeolog, anticorps SMURF2, anticorps smurf2, anticorps Smurf2, anticorps smurf2.S

Sujet:

Synonyms: E3 ubiquitin-protein ligase SMURF2, hSMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF2

Background: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

ID gène: 64750

UniProt: Q9HAU4