AKT 1/2/3 anticorps (pTyr312, pTyr315, pTyr316)

N° du produit ABIN756217

Détail du produit anti-AKT 1/2/3 anticorps

Antigène: AKT 1/2/3

Épitope: pTyr312, pTyr315, pTyr316

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AKT 1/2/3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This antibody will detect AKT 1 when phosphorylated at Tyr315 in Human, Mouse, Rat or Bovine, AKT2 when phosphorylated at Tyr316 in Human, Mouse, Rat, or Bovine, and AKT3 when phosphorylated at Tyr312 in Human, Mouse, Rat, or Bovine.

Réactivité croisée: Humain, Souris, Rat

Homologie: Dog,Cow,Sheep,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Tyr315

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-AKT 1/2/3 (ABIN756217)

Iriyama, Hatta, Takei: "Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes." dans: Cancer medicine, Vol. 5, Issue 11, pp. 3223-3234, (2016) (PubMed).

Détails sur AKT 1/2/3

Antigène: AKT 1/2/3

Autre désignation: AKT1+2+3 (AKT 1/2/3 Produits)

Sujet:

Synonyms: AKT1/AKT2/AKT3 Tyr315+Tyr316+Tyr312, AKT1/AKT2/AKT3Tyr315/316/312, AKT1/2/3 phospho Y315 + Y316 + Y312, AKT 1, AKT, AKT1, AKT-1, AKT1_HUMAN, AKT 2, AKT2, AKT-2, AKT2_HUMAN, AKT 3, AKT3, AKT-3, AKT3_HUMAN, C AKT, cAKT, MGC9965, MGC99656, Oncogene AKT1, PKB, PKB alpha, PKB-ALPHA, PKB beta, PKB gamma, PRKBA, Protein Kinase B Alpha, Protein kinase B, Proto-oncogene c-Akt, RAC Alpha, RAC alpha serine/threonine protein kinase, RAC, RAC PK Alpha, Rac protein kinase alpha, RAC Serine/Threonine Protein Kinase, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, v akt murine thymoma viral oncogene homolog 1, vAKT Murine Thymoma Viral Oncogene Homolog 1.

Background: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82 % identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.

ID gène: 207, 208, 10000