WEE1 anticorps (pSer123)

N° du produit ABIN756592

Détail du produit anti-WEE1 anticorps

Antigène: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Épitope: pSer123

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WEE1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This phosphorylation site is homologous to Ser123 in Mouse, Rat, and Bovine. The peptide used to produce this antibody has high homology with a sequence occurring in MIER3, and may react with this protein in Mouse and Human when phosphorylated at the site of Ser10.

Réactivité croisée: Souris

Homologie: Human,Rat,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human Wee1 around the phosphorylation site of Ser123 [SS(p-S)PV]

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur WEE1

Antigène: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Autre désignation: Wee1 (WEE1 Produits)

Synonymes: anticorps WEE1A, anticorps WEE1hu, anticorps ATWEE1, anticorps F22D16.3, anticorps F22D16_3, anticorps WEE1 kinase homolog, anticorps WEE1-KINASE, anticorps WEE1, anticorps Xe-Wee1B, anticorps XeWee1B, anticorps wee1-b, anticorps wee1a, anticorps wee2-a, anticorps xe-wee1a, anticorps xwee1, anticorps wee1, anticorps wee1b, anticorps wee1hu, anticorps xwee2, anticorps GB19689, anticorps Wee1A, anticorps fa20h03, anticorps wu:fa20h03, anticorps zgc:136514, anticorps WEE1 G2 checkpoint kinase, anticorps Wee1 kinase, anticorps WEE1-like kinase, anticorps WEE1 G2 checkpoint kinase S homeolog, anticorps wee1 homolog, anticorps WEE1 homolog 2, anticorps wee1-like protein kinase, anticorps WEE 1 homolog 1 (S. pombe), anticorps M phase inhibitor protein kinase Wee1, anticorps WEE1, anticorps Wee1, anticorps wee1.S, anticorps wee1-a, anticorps wee2, anticorps LOC551677, anticorps wee1

Sujet:

Synonyms: WEE1A, WEE1hu, Wee1-like protein kinase, Wee1A kinase, WEE1

Background: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

ID gène: 7465

UniProt: P30291

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases, M Phase